evaluation of human leukocyte antigen-g and interleukin-10 expression in hiv positive patients and healthy subjects

نویسندگان

ورسا عمرانی نوا

versa omrani-nava msc student in immunology, student research committee, faculty of medicine, mazandaran university of medical sciences, sari, iranدانشجوی کارشناسی ارشد ایمونولوژی، کمیته تحقیقات دانشجویی، دانشکده پزشکی، دانشگاه علوم پزشکی مازندران، ساری، ایران سعید عابدیان کناری

saeid abediankenari professor, immunogenetics research center, mazandaran university of medical sciences, sari, iranساری: دانشگاه علوم پزشکی مازندران- دانشکده پزشکی جمشید یزدانی چراتی

jamshid yazdani charati associate professor, department of biostatistics, health sciences research center, mazandaran university of medical sciences, sari, iranدانشیار، گروه آمار زیستی، مرکز تحقیقات علوم بهداشتی، دانشگاه علوم پزشکی مازندران، ساری، ایران یوسف یحیی پور

yousef yahyapour assistant professor, department of microbiology, infectious diseases and tropical medicine research center, babol university of medical sciences, babol, iranاستادیار، گروه میکروبیولوژی، مرکز تحقیقات بیماری های عفونی و گرمسیری، دانشگاه علوم پزشکی بابل، بابل، ایران سعیده سادات شبیری

چکیده

background and purpose: hila-g is an immunomodulatory molecule and alteration of its expression is prevalent during viral infections. hla-g expression in hiv-positive patients has been inconsistent between different studies and some suggest that interleukin-10 (il-10) influences the expression of hla-g. the aim of this research was to investigate the expression of il-10 in hiv-positive patients and its relation with tcd4+ lymphocytes. materials and methods: in a case-control study, 20 hiv-positive patients, attending the behavioral consultation center affiliated with mazandaran university of medical sciences, were selected and 20 healthy volunteers were also enrolled. expression of hla-g was determined by real-time pcr. tcd4+ count and il-10 concentration were evaluated by flow cytometry and elisa, respectively. results: hla-g gene expression was significantly different between the two groups (p<0.05). il-10 concentration increased in patients and a revers relation was seen between tcd4+ cell count and il-10 (r= -0.4, p= 0.1). conclusion: the results showed that the expression of hla-g is deregulated during hiv infection and il-10 could be considered as a biomarker of disease progression.

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عنوان ژورنال:
مجله دانشگاه علوم پزشکی مازندران

جلد ۲۶، شماره ۱۴۳، صفحات ۲۳۰-۲۳۴

کلمات کلیدی
[ ' h l a ' , ' g ' , ' h i v ' , ' i l ' , 1 0 ]

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